pbluescriptiiks(+) (genbank accession no. x52327) (Agilent technologies)
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Pbluescriptiiks(+) (Genbank Accession No. X52327), supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pbluescriptiiks/pmc04045853-59-1-8
Average 90 stars, based on 1 article reviews
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Gene Expression:Article Title: Antibody diagnostic kits and methods of use Article Snippet: .. Gene Expression in Mouse Embryos In order to generate an antisense RNA probe for mouse VEGF-D mRNA, the mouse VEGF-D2 cDNA shown in FIG. 7 (SEQ ID NO:7) was inserted into the Article Title: Methods of making VEGF-D polypeptides Article Snippet: .. Gene Expression in Mouse Embryos In order to generate an antisense RNA probe for mouse VEGF-D mRNA, the mouse VEGF-D2 cDNA shown in FIG. 7 (SEQ ID NO:7) was inserted into the Article Title: Methods of stimulating wound healing by administration of vascular endothelial growth factor D (VEGF-D) Article Snippet: .. Gene Expression in Mouse Embryos In order to generate an antisense RNA probe for mouse VEGF-D mRNA, the mouse VEGF-D2 cDNA shown in FIG. 7 (SEQ ID NO:7) was inserted into the Plasmid Preparation:Article Title: Antibody diagnostic kits and methods of use Article Snippet: .. Gene Expression in Mouse Embryos In order to generate an antisense RNA probe for mouse VEGF-D mRNA, the mouse VEGF-D2 cDNA shown in FIG. 7 (SEQ ID NO:7) was inserted into the Article Title: Methods of making VEGF-D polypeptides Article Snippet: .. Gene Expression in Mouse Embryos In order to generate an antisense RNA probe for mouse VEGF-D mRNA, the mouse VEGF-D2 cDNA shown in FIG. 7 (SEQ ID NO:7) was inserted into the Article Title: Methods of stimulating wound healing by administration of vascular endothelial growth factor D (VEGF-D) Article Snippet: .. Gene Expression in Mouse Embryos In order to generate an antisense RNA probe for mouse VEGF-D mRNA, the mouse VEGF-D2 cDNA shown in FIG. 7 (SEQ ID NO:7) was inserted into the Southern Blot:Article Title: Genetic Background and Mobility of Variants of the Gene nleA in Attaching and Effacing Escherichia coli Article Snippet: .. Finally, the phage DNA was dissolved in 100 μl of distilled water, restricted with EcoRI overnight, and analyzed by Southern blot hybridization ( 7 ), and nleA -positive DNA fragments were cloned in the Hybridization:Article Title: Genetic Background and Mobility of Variants of the Gene nleA in Attaching and Effacing Escherichia coli Article Snippet: .. Finally, the phage DNA was dissolved in 100 μl of distilled water, restricted with EcoRI overnight, and analyzed by Southern blot hybridization ( 7 ), and nleA -positive DNA fragments were cloned in the Clone Assay:Article Title: Genetic Background and Mobility of Variants of the Gene nleA in Attaching and Effacing Escherichia coli Article Snippet: .. Finally, the phage DNA was dissolved in 100 μl of distilled water, restricted with EcoRI overnight, and analyzed by Southern blot hybridization ( 7 ), and nleA -positive DNA fragments were cloned in the Article Title: Application of Trichoderma reesei cellulase and xylanase promoters through homologous recombination for enhanced production of extracellular beta-glucosidase I. Article Snippet: One of the limiting factors for the application of Trichoderma reesei to degrade cellulosic biomass is its low -glucosidase activity, required to convert cellobiose to glucose.. The egl3 and the xyn3 promoters were used to express -glucosidase 1 gene bgl1 through homologous recombination to improve the cellulose degradation ability of T. reesei.. The recombinant strains expressing -glucosidase 1 (BGLI) under the control of either the egl3 or the xyn3 promoter had 4.0 and 7.5 fold higher -glucosidase activity than the native strain, which compares well to the finding that in wild-type T. reesei PC-3-7, the levels of egl3 and xyn3 mRNA expression were 6.0 and 12 fold higher respectively than that of bgl1. other:Article Title: Frequency and diversity of small cryptic plasmids in the genus Rahnella Article Snippet: Different types of plant materials (Table ) were homogenised in sterile PBS and dilutions plated on Levine-EMB agar (Merck, Darmstadt, Germany). Transformation Assay:Article Title: The plant‐parasitic cyst nematode effector GLAND4 is a DNA‐binding protein Article Snippet: .. After the final three rounds, an aliquot of the eluted DNA was ligated into the Not I site of |
